Immobilization of Kluyveromyces marxianus cells with inulinase in agar.
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چکیده
منابع مشابه
Production of inulinase from Kluyveromyces marxianus using dahlia tuber extract
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...
متن کاملOPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus
Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett– –Burman design...
متن کاملEffects of Culture Conditions on the Production of Inulinase by Kluyveromyces marxianus
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL) was obtained at a sucrose concentration of 10 g L, pH 5.0 and aeration rate of 1 vvm. The 20 g L sucrose concentration was suitable for...
متن کاملStructure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556.
In the yeast Kluyveromyces marxianus two forms of inulinase were present, namely, an inulinase secreted into the culture fluid and an inulinase retained in the cell wall. Both forms were purified and analyzed by denaturing and nondenaturing polyacrylamide gel electrophoresis. With the use of endo-beta-N-acetyl-glucosaminidase H, it was established that the enzyme retained in the cell wall and t...
متن کاملEffects of carbon sources, oxygenation and ethanol on the production of inulinase by Kluyveromyces marxianus YX01
Article info: Received: 16 July 2012 In revised form: 25 September 2012 Accepted: 29 October 2012 ABSTRACT Inulinase is one of the most important factors in consolidated bioprocessing, which combines enzyme production, inulin saccharification, and ethanol fermentation into a single process. In our study, inulinase production and cell growth of Kluyveromyces marxianus YX01 under different condit...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1985
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.31.297